Strategies for reducing noxious gas emissions in pig production: a comprehensive review on the role of feed additives.

The emission of noxious gases is a significant problem in pig production, as it can lead to poor production, welfare concerns, and environmental pollution. The noxious gases are the gasses emitted from the pig manure that contribute to air pollution. The increased concentration of various harmful gasses can pose health risks to both animals and humans. The major gases produced in the pig farm include methane, hydrogen sulfide, carbon dioxide, ammonia, sulfur dioxide and volatile fatty acids, which are mainly derived from the fermentation of undigested or poorly digested nutrients. Nowadays research has focused on more holistic approaches to obtain a healthy farm environment that helps animal production. The use of probiotics, prebiotics, dietary enzymes, and medicinal plants in animal diets has been explored as a means of reducing harmful gas emissions. This review paper focuses on the harmful gas emissions from pig farm, the mechanisms of gas production, and strategies for reducing these emissions. Additionally, various methods for reducing gas in pigs, including probiotic interventions; prebiotic interventions, dietary enzymes supplementation, and use of medicinal plants and organic acids are discussed. Overall, this paper provides a comprehensive review of the current state of knowledge on reducing noxious gas in pigs and offers valuable insights for pig producers, nutritionists, and researchers working in this area.

Dietary eubiotics of microbial muramidase and glycan improve intestinal villi, ileum microbiota composition and production trait of broiler.

BACKGROUND: Optimal gut health is important to maximize growth performance and feed efficiency in broiler chickens. A total of 1,365 one-day-old male Ross 308 broiler chickens were randomly divided into 5 treatments groups with 21 replicates, 13 birds per replicate. The present research investigated effects of microbial muramidase or a precision glycan alone or in combination on growth performance, apparent total tract digestibility, total blood carotenoid content, intestinal villus length, meat quality and gut microbiota in broiler chickens. Treatments included: NC: negative control (basal diet group); PC: positive control (basal diet + 0.02% probiotics); MR: basal diet + 0.035% microbial muramidase; PG: basal diet + 0.1% precision glycan; and MRPG: basal diet + 0.025% MR + 0.1% PG, respectively. RESULTS: MRPG group increased the body weight gain and feed intake (P < 0.05) compared with NC group. Moreover, it significantly increased total serum carotenoid (P < 0.05) and MRPG altered the microbial diversity in ileum contents. The MRPG treatment group increased the abundance of the phylum Firmicutes, and family Lachnospiraceae, Ruminococcaceae, Oscillospiraceae, Lactobacillaceae, Peptostreptococcaceae and decreased the abundance of the phylum Campilobacterota, Bacteroidota and family Bacteroidaceae. Compared with the NC group, the chickens fed MRPG showed significantly increased in duodenum villus length at end the trial. CONCLUSION: In this study, overall results showed that the synergetic effects of MR and PG showed enhancing growth performance, total serum carotenoid level and altering gut microbiota composition of broilers. The current research indicates that co-supplementation of MR and PG in broiler diets enhances intestinal health, consequently leading to an increased broiler production.

Effects of dietary supplementation of illite on growth performance, nutrient digestibility, and meat-carcass grade quality of growing-finishing pigs.

This study investigated the impact of clay mineral 'illite' (IL) on the growth performance, apparent total tract digestibility, and meat-carcass grade quality in growing-finishing pigs. One hundred fifty pigs were divided into two groups with fifteen pens/group and five pigs/pen, a control group that was fed with a corn-soybean meal-based diet and IL treated group were fed a meal-based diet supplemented with 0.5% IL. Compared to the control, IL supplementation increased average daily feed intake (ADFI) and reduce gain to feed ratio (G:F) in the pigs during days 43 to 70 and 99 to 126 and increased dry matter during days 42 and 126, and backfat thickness on day 98 (p < 0.05). The drip loss was reduced on day 7, meat firmness tended to increase with dietary IL supplementation. In summary, dietary 0.5% IL supplementation improved ADFI, nutrient utilization of dry matter, and firmness and reduced G:F and drip loss of growing-finishing pigs.

Comparative effects of dietary herbal mixture or guanidinoacetic acid supplementation on growth performance, cecal microbiota, blood profile, excreta gas emission, and meat quality in Hanhyup-3-ho chicken.

Phytogenic feed additives are renowned for their growth promotion, gut health enhancement, and disease prevention properties, which is important factors for sustaining prolonged poultry rearing. The study aimed to evaluate the effect of herbal mixture (mixture of ginseng and artichoke) or guanidinoacetic acid (GAA) on growth performance, cecal microbiota, excretal gas emission, blood profile, and meat quality in Hanhyup-3-ho chicken. A total of 360 one-day-old chickens (half males and half females) were allocated into one of 3 dietary treatments (12 replicate cages/treatment; 10 broilers/replicate cage) for 100 d of age. Experimental diets were CON: basal diet; TRT1: basal diet combined with 0.05% herbal mixture; and TRT2: basal diet combined with 0.06% GAA. All birds received a basal diet during the first 30 d, but from d 31 to 100, an experimental diet was supplied. The addition of 0.05% herbal mixture improved the average body weight gain and feed conversion ratio from d 31 to 100 as well as the overall experimental period. The cecal Lactobacillus, Escherichia coli, and Salmonella count remained consistent across all dietary treatments. Blood albumin and Superoxide Dismutase (SOD) levels increased in the herbal mixture supplemented diet. Additionally, there was a notable reduction in excretal NH3 and H2S emissions in the herbal mixture group. Furthermore, the herbal mixture group exhibited increased breast muscle weight, improved breast muscle color, improved water holding capacity, and a decrease in abdominal fat compared to the control group. Additionally, the supplementation of 0.06% GAA did not demonstrate any statistically significant impact on any evaluated parameter throughout the experiment. The results from the present investigation underscore the potential of ginseng together with artichoke extract supplementation as a viable feed additive, conferring improvements in growth performance, feed efficiency, excreta gas emission, meat quality parameters, and defense mechanism against oxidative stress in Hanhyup-3-ho chicken.

Probiotic, Paraprobiotic, and Hydrolyzed Yeast Mixture Supplementation Has Comparable Effects to Zinc Oxide in Improving Growth Performance and Ameliorating Post-weaning Diarrhea in Weaned Piglets.

A total of 150 21-day-old weaned piglets [(Yorkshire × Landrace) × Duroc] were randomly assigned to 3 groups (CON, TRT1, TRT2) to evaluate the effects of dietary supplementation of probiotic, paraprobiotic, and hydrolyzed yeast mixture (PPY) on growth performance, nutrient digestibility, fecal bacterial counts, fecal calprotectin contents, and diarrhea rate in a 42-day experiment (phase 1: days 1-14; phase 2: days 15-42). There were 10 replicate pens per treatment with 5 pigs per pen (three gilts and two barrows). Pigs in CON were only provided with a basal diet. Pigs in TRT1 were provided with a basal diet + 3000 mg/kg zinc oxide during phase 1 and a basal diet during phase 2. Pigs in TRT2 were provided with a basal diet + 200 mg/kg probiotic (Saccharomyces cerevisiae boulardii) + 800 mg/kg paraprobiotic (inactivated yeast strains of Saccharomyces cerevisiae and Cyberlindnera jadinii) + 10 g/kg hydrolyzed yeast mixture during phase 1, and a basal diet + 100 mg/kg probiotic + 400 mg/kg paraprobiotic mixture during phase 2. Pigs in TRT1 and TRT2 were significantly heavier at day 14 and 42 than CON pigs. Growth rate during days 1-14, 15-42, and 1-42 and feed efficiency during days 1-14 were similarly affected by treatment while feed efficiency was significantly higher for TRT2 pigs between 15-42 and 1-42 days. Moreover, nitrogen and energy digestibility in both TRT1 and TRT2 were higher than that in CON. During experimental periods, diarrhea rate in TRT1 and TRT2 was lower than that in CON. Therefore, we demonstrated that PPY supplementation had comparable effects as ZnO in improving growth performance and nutrient digestibility as well as ameliorating post-weaning diarrhea in weaned piglets.

Pinecone oil supplemented to multiparous sows from 107 days prenatal to 21 days postpartum improves reproductive performance and milk composition and affects serum parameters.

Pinecone oil (PO) of Pinus koraiensis mainly contains α-pinene, ß-pinene, and limonene that may ameliorate animal well-being and growth performance. This study evaluated its effects on feed intake, milk composition and yield, serum parameters, and litter growth of sows. Twenty-seven pregnant sows (parity 2-4) were distributed to three dietary treatments. The trial started on Day 107 of gestation and ended on Day 21 of lactation. Sows were given either a basal diet or the basal diet + 200 or 400 mg/kg PO. Each treatment contained nine sows and each sow was considered an experimental unit. Results showed that the average daily gain and weaned body weight of piglets from the sows fed 400 mg/kg PO supplements were higher (p < 0.05) than the piglets from the control sows. Lactose content in colostrum samples and fat content in milk samples were higher (p < 0.05) in 400 mg/kg PO-treated sows, respectively, than those from the sows fed basal diet. Additionally, cortisol concentration and aspartate aminotransferase concentration in sow serum was lowered (p < 0.05) by 400 mg/kg PO on Day 21 of lactation. In conclusion, supplementation of 400 mg/kg PO during late gestation and lactation contributed to greater offspring growth performance, possibly by enhanced milk quality and alleviated maternal stress.

Influences of flavonoid (quercetin) inclusion to corn-soybean-gluten meal-based diet on broiler performance.

Quercetin (a predominant flavonoid) is considered to have antimicrobial and antioxidant properties. This trial was conducted to evaluate the impact of graded doses of quercetin (QS) on growth efficiency, nutrient retention, faecal score, footpad lesion score, tibia ash and meat quality. In a 32-day feeding test, a total of 576 1-day-old Ross 308 broilers (male) were allocated arbitrarily with an average body weight of 41 ± 0.5 g. The trial had four dietary treatments with eight repetitions of 18 birds per pen and a basal diet incorporating 0%, 0.02%, 0.04% and 0.06% of QS. As the QS dosage increased, body weight gain tended to increase linearly on Days 9-21 (p = 0.069) and overall period (p = 0.079). Similarly, feed intake increased (p = 0.009) linearly with the increasing doses of QS on Days 9-21. Likewise, there was a linear improvement in dry matter (p = 0.002) and energy (p = 0.016) digestibility after QS administration. Moreover, the inclusion of QS supplement (0%-0.06%) linearly increased (p = 0.012) tibia ash in broilers. However, the faecal score and footpad lesion score showed no significant outcome (p > 0.05). By giving broilers a graded amount of QS, the relative organ weights of breast muscle (p = 0.009) and spleen (p = 0.006) improved linearly, meat colour lightness increased (p = 0.015), redness tended to improve (p = 0.065) linearly and drip loss decreased (p = 0.015) linearly. The inclusion of QS in the graded-level diet led to improvements in growth efficiency, nutrient absorption, meat quality and tibia ash, which recommended it as a beneficial feed additive for the broiler.

Assessment of a 50:50 mixture of two Bacillus subtilis strains as growth promoters for finishing pigs: productivity improvement and noxious gas reduction.

In this study, we aimed to assess the potential of a 50:50 mixture of two Bacillus subtilis strains in improving the productivity and health of finishing pigs and reducing noxious gases in their feces. These strains were found to abundantly secrete surfactin which has been shown to alleviate the effects of lipopolysaccharides in vitro. For the 10-wk experiment, 200 finishing pigs ([Landrace × Yorkshire] × Duroc) with an average body weight of 54.15 ±â€…1.70 kg were divided into four groups. Each group was fed with a basal diet supplemented with an equal amount of spores from the two B. subtilis strains at different levels: control group, no addition; treatment group 1, 0.5 × 109; treatment group 2, 1.0 × 109; treatment group 3, 1.5 × 109 cfu·kg-1 addition. During the 10-wk feeding period, dietary supplementation of 0.5 × 109, 1.0 × 109, and 1.5 × 109 cfu·kg-1 of the spore cells from these two strains resulted in a 0.9%, 1.9%, and 2.5% increase in body weight, respectively (linear P < 0.095). During the final 5 wk, the average daily gain (ADG) in weight was increased by the strains at amounts of 0.5 × 109, 1.0 × 109, and 1.5 × 109 cfu·kg-1 with a clear dosage effect (linear P < 0.05). However, neither the gain-to-feed ratio, the average daily feed intake, nor nutrient digestibility was affected by the supplementation. In blood, the endotoxin lipopolysaccharides, and two liver toxicity indicator enzymes; aspartate aminotransferase and lactate dehydrogenase were decreased (P < 0.05) in the 1.0 × 109 cfu·kg-1 spores-feeding group. Furthermore, four noxious gases were reduced by 8 to 20% in feces excreted by pigs fed with 1.5 × 109 cfu·kg-1 spores with a linear dosage effect (linear P < 0.001 to 0.05) during the final 5 wk. Our findings suggest that the mixture of B. subtilis strains may enhance the productivity of finishing pigs by reducing the risk of mild endotoxemia, rather than increasing digestibility or daily feed intake. Therefore, these Bacillus strains have the potential to act as growth promoters for pigs, leading to improved animal health and productivity. These results have significant implications for pig farmers seeking to optimize the health and growth of their animals.

In a previous study, we discovered two new strains of Bacillus subtilis that showed high surfactin secretion during growth in culture media. This surfactin proved effective in reducing endotoxin effects, particularly lipopolysaccharides, in vitro. To explore their potential as pig growth promoters, we administered 50:50 bacteria blend to 200 finishing pigs, dividing them into four groups for a 10-wk trial. Results showed that supplementing the pigs' diet with 0.5, 1.0, or 1.5 billion bacteria per kilogram led to weight gains of 0.9%, 1.9%, and 2.5%, respectively, with a dosage effect. The weight gain was notably higher during the final 5 wk. However, there were no significant differences in feed intake or nutrient digestibility. Blood analysis revealed reduced lipopolysaccharides and liver toxicity indicators, suggesting improved animal health. Moreover, the pigs that received the bacterial mixture showed reduced noxious gas levels in their feces with a dosage effect. These findings suggest that these new B. subtilis strains could serve as effective growth promoters for pigs by minimizing the risk of mild endotoxemia, leading to enhanced animal health and productivity. These results could have valuable implications for pig farmers seeking to optimize the health and growth of their animals.

Investigating the Chemical Composition of Lepidium sativum Seeds and Their Ability to Safeguard against Monosodium Glutamate-Induced Hepatic Dysfunction.

Monosodium glutamate (MSG) is one of the most frequently used food additives that endanger public health. The antioxidant, hyperlipidemic, and cytoprotective properties of Lepidium sativum seeds (LSS) as a natural remedy can minimize the harmful effects of MSG. This study investigated the potential protective effect of LSS against MSG-induced hepatotoxicity in rats. Male albino Sprague Dawley rats (n = 24) were equally divided into four groups for 30 days: the control group (G1) received a basal diet without supplement, group (G2) was fed a basal diet + MSG (30 g/kg b.w.) as a model group, group (G3) was fed a basal diet + MSG (30 g/kg b.w.) + LSS (30 g/kg b.w.), and group (G4) was fed a basal diet + MSG (30 g/kg b.w.) + LSS (60 g/kg b.w.). LSS enhanced serum alkaline phosphatase activity as well as total cholesterol, triglyceride, and glucose levels. It can decrease peroxide content in serum lipids and inhibit glutathione reductase and superoxide dismutase in hepatic cells. The dietary supplementation with LSS provided cytoprotection by enhancing the histoarchitecture of the liver and decreasing the number of apoptotic cells. Due to their antioxidant and anti-apoptotic properties, LSS effectively protect against the hepatotoxicity of MSG. These findings are of the highest significance for drawing attention to incorporating LSS in our food industry and as a health treatment in traditional medicine to combat MSG-induced hepatic abnormalities.

The Efficacy of Yeast Supplementation on Monogastric Animal Performance-A Short Review.

Due to a continual growth in the world's population and the prohibition of antibiotics in animal production, the livestock industry faces significant challenges in the global demand for meat, eggs, and dairy products. The growing demand for organic products and the prohibition on antibiotic growth promoters (AGPs) have compelled animal nutrition experts to search for natural substitutes that include medical plants and beneficial microorganisms. Natural feed additives like probiotics are found to be more effective than AGPs in reducing the load of harmful intestinal pathogens. One of the probiotics that has generated considerable interest since ancient times is yeast. Yeast is used as a supplement in animal feeds due to its relatively high protein, amino acid, energy, and micronutrient content. Yeast byproducts such as yeast cells and cell walls contain nutraceutical compounds (i.e., ß-glucans, mannooligosaccharides, and nucleotides) and have been shown to improve animal growth performance and health. Though the application of yeast supplements has been reviewed to date, only a scarce amount of information exists on the yeast-derived products in non-ruminant nutrition. Additionally, it is difficult for nutritionists to differentiate the characteristics, composition, and optimal feeding among the diverse number of yeast-containing products. Due to the increasing popularity of using yeast-based products in animal feeds, the development of analytical approaches to estimate yeast and its components in these products is greatly needed. Thus, in this review, we intend to provide current knowledge of different categories of commercially available yeast and yeast-derived additives, along with their role in improving animal growth performance and health, their proposed mechanisms of action, and the challenges of quantifying yeast content and biologically active components.

Effect of dietary supplementation of betaine and organic minerals on growth performance, serum biochemical parameters, nutrients digestibility, and growth-related genes in broilers under heat stress.

Global warming and climate changes have a detrimental impact on poultry production, causing substantial economic losses. This study investigated the effects of incorporating dietary betaine (BT) and organic minerals (OMs) on broilers' performance as well as their potential to mitigate the negative impacts of heat stress (HS). Six hundred 1-day-old Ross 308 chicks were randomly allocated to 12 experimental treatments with 5 replicates of 10 birds each (5 male + 5 female). The birds were provided with diets containing BT (0 and 2,000 ppm) and OMs (0, 250, and 500 ppm), either individually or in combination, under both thermoneutral and HS-inducing temperatures. The HS conditions involved exposing the birds to cyclic periods of elevated temperature (35°C ± 2°C) for 6 h daily, from 10:00 am to 4:00 pm, starting from d 10 and continuing until d 35. The exposure to HS deteriorated birds' growth performance; however, dietary BT and OMs inclusion improved the growth performance parameters bringing them close to normal levels. Carcass traits were not affected by dietary supplementation of BT, OMs, HS, or their interaction. Interestingly, while HS led to increased (P < 0.05) levels of total cholesterol, LDL-cholesterol, and hepatic malondialdehyde (MDA), these adverse effects were mitigated (P < 0.05) by the addition of BT and OMs. Moreover, dietary BT supplementation led to elevated serum total protein and globulin concentrations. Cyclic HS did not alter Mn, Zn, and Cu contents in the pectoral muscle. However, the incorporation of OMs at both levels increased concentrations of these minerals. Notably, the combination of 500 ppm OMs and 2,000 ppm BT improved Mn, Zn, Cu, and Fe digestibility, which has been compromised under HS conditions. Cyclic HS upregulated gene expression of interleukin-1ß, heat shock protein 70, and Toll-like receptor-4 while downregulated the expression of claudin-1, uncoupling protein, growth hormone receptor, superoxide dismutase 1, glutathione peroxidase 1 and insulin-like growth factor 1. The aforementioned gene expressions were reversed by the combination of higher dietary levels of BT and OMs. In conclusion, the dietary supplementation of 500 ppm OMs along with 2,000 ppm BT yielded significant improvements in growth performance and mineral digestibility among broiler chickens, regardless of thermal conditions. Moreover, this combination effectively restored the expression of growth-related genes even under heat-stress conditions.

Dietary exogenous phytase improve egg quality, reproductive hormones, and prolongs the lifetime of the aging Hy-Line brown laying hens fed nonphytate phosphorus.

The study examined how adding phytase to nonphytate phosphorus (NPP) diets affected performance, egg quality, reproductive hormones, and plasma biochemical indices in 73- to 80-wk-old laying hens. Six treatments with 5 replicates of 18 Hy-Line brown laying hens each were randomly assigned. Three isonitrogenous, isocaloric diets containing consistent calcium levels (3.8%) were formulated to contain 0.20, 0.25, and 0.30% NPP, treated with or without phytase supplementation (1,000 FYT per kg feed, Ronozyme HiPhos-L, Aspergillus oryzae 6-phytase). The results showed that the addition of phytase to the diet containing 0.20, 0.25, and 0.30% NPP increased egg production by 1.50, 1.64, and 0.97%, respectively, and improved eggshell thickness. Also, use of phytase in the diet contain 0.25, and 0.30% NPP increased the plasma concentration of albumin (ALB), high-density lipoprotein (HDL), phosphorus (P), and plasma follicle-stimulating hormone (FSH), plasma calcium (Ca), estradiol-17ß (E2ß), and luteinizing hormone (LH). In contrast, the egg weight, feed intake, egg mass, feed conversion ratio, albumen height, Haugh unit, yolk, and shell color were unaffected. It can be advisable to use phytase supplementation in an elderly laying hen's diet contain 0.25, and 0.30% NPP to improve shell quality and positively impact reproductive hormones leading to the persistence of egg production.

Effects of herbal supplements on milk production quality and specific blood parameters in heat-stressed early lactating cows.

The present study explored the influence of supplemental herbal mixtures on cow milk production, quality, and blood parameters in dairy cows under high ambient temperatures. Thirty Holstein cows were randomly assigned into three experimental groups of 10 each. The first control group was supplied with the commercial basal diet, whereas two treatment groups were provided with the commercial basal diet supplemented with 50 and 100 g/head/day of the herbal mixture, respectively. The results showed that the mixture of herbal supplementation did not influence weekly milk production. Milk total fat, triglyceride, and total protein values were not affected (p < 0.05) in cows fed on basal diets supplemented with herbal mixture; however, milk cholesterol was decreased significantly by 100 mg/head/day of the herbal mixture. On the other hand, lactose has increased significantly by adding 100 mg/head/day of herbal mixture. Furthermore, the total cholesterol level in serum was decreased by adding 100 mg/head/day of the herbal mixture, while plasma prolactin, cortisol, GOT, and GPT were unaffected. Regarding fatty acids (C18, C18:1 (c9), 18:1 (c11), 18:2 (c9, c12), 18:2 (t9, t12), and CLA (c9, t11)), there was no significant variation between the groups. Meanwhile, both C19:00 and 18:3 (c6, c9, and c12) were noticeably higher (p < 0.05) in the group that received 100gm, followed by 50 mg, compared to the control. In conclusion, the supplement with a herbal mixture positively affected milk quality by decreasing total cholesterol and increasing lactose, milk fatty acid profile by increasing unsaturated fatty acids content, and plasma cholesterol levels.

An evaluation of gallic acid supplementation to corn-soybean-gluten meal-based diet in broilers.

Gallic acid (GA) is an endogenous plant polyphenol found in fruits, nuts, and plants that has antioxidant, antimicrobial, and growth-promoting effects. This study aimed to assess the effect of graded doses of dietary supplemented GA on growth performance, nutrient retention, fecal score, footpad lesion score, tibia ash, and meat quality of broilers. A total of 576 one-day-old Ross 308 male broiler chicks with an average initial body weight of 41 ± 0.5 g were used in a 32 d feeding trial. Broilers were sorted into 4 treatments, 8 replications per treatment, and 18 birds per cage. Dietary treatments consisted of corn-soybean-gluten meal-based basal diet and the basal diet supplemented with 0, 0.02, 0.04, and 0.06% of GA. Feeding broilers with a graded doses of GA increased body weight gain (BWG) (P < 0.05) and feed intake (P < 0.05) linearly on phase 2 (d 9-21). Additionally, the nutrient digestibility of dry matter (P < 0.05) and energy (P < 0.05) was increased linearly by including a rising level of GA in the broiler diet. However, the excreta score, footpad lesion score, tibia ash, and meat quality presented no significant effect (P > 0.05) except meat color of yellowness. Adding GA at increasing doses to broiler diets increased growth efficiency and nutritional absorption without affecting excreta score, footpad lesion score, tibia ash, and meat quality. In conclusion, the inclusion of graded levels of GA to corn-soybean-gluten meal-based diet presented dose-dependent improvement in growth performance and nutrient digestibility of broilers.

Effects of fermented soybean meal supplementation on the growth performance and apparent total tract digestibility by modulating the gut microbiome of weaned piglets.

This study investigates the effects of soybean meal fermented by Enterococcus faecium as a replacement for soybean meal on growth performance, apparent total tract digestibility, blood profile and gut microbiota of weaned pigs. Eighty piglets (weaned at 21 days) [(Landrace × Yorkshire) × Duroc] with average body weight of 6.52 ± 0.59 kg) were selected and assigned to 4 treatments/4 replicate pens (3 barrows and 2 gilts). The four diets (SBM, 3, 6 and 9% FSBM) were formulated using fermented soybean meal to replace 0, 3, 6 and 9% of soybean meal, respectively. The trial lasted for 42 days phase 1, 2 and 3. Result showed that supplemental FSBM increased (P < 0.05) the body weight gain (BWG) of piglets at day 7, 21 and 42 and ADG at days 1-7, 8-21, 22-42 and 1-42, and ADFI at days 8-21, 22-42 and 1-42 and G: F at days 1-7, 8-21 and 1-42, and crude protein, dry matter, and gross energy digestibility at day 42, and lowered (P < 0.05) diarrhea at days 1-21 and 22-42. The concentration of glucose levels, WBC, RBC, and lymphocytes were increased while, concentration of BUN level in the serum was lowered in the FSBM treatment compared to the SBM group (P < 0.05). Microbiota sequencing found that FSBM supplementation increased the microbial Shannon, Simpsons and Chao indexs, (P < 0.05) and the abundances of the phylum Firmicutes, and genera prevotella, Lactobacillus, Lachnospiraceae and Lachnoclostridium (P < 0.05), lower in the abundances of the phylum bacteroidetes, Proteobacteria, genera Escherichia-Shigella, Clostridium sensu stricto1, Bacteroides and Parabacteroides (P < 0.05). Overall, FSBM replacing SBM improved the growth performance, apparent total tract digestibility, and blood profiles; perhaps via altering the faecal microbiota and its metabolites in weaned pigs. The present study provides theoretical support for applying FSBM at 6-9% to promote immune characteristics and regulate intestinal health in weaning piglets.

Evaluation on the Growth Performance, Nutrient Digestibility, Faecal Microbiota, Noxious Gas Emission, and Faecal Score on Weaning Pigs Supplement with and without Probiotics Complex Supplementation in Different Level of Zinc Oxide.

A total of 200 26-day-old crossbred weaning piglets ((Yorkshire × Landrace) × Duroc; 6.55 ± 0.62 kg) were used in a 6-week experiment to evaluate the effects of adding probiotics complex supplementation (Syner-ZymeF10) with high and low ZnO diets on the performance of weaning pigs in 42 days. Pigs were randomly allotted to a 2 × 2 factorial arrangement and they were supplemented with two concentration level of ZnO with 3000 ppm and 300 ppm and probiotics complex supplementation with 0 and 0.1%. There were ten replicate pens per treatment with five pigs per pen (two gilts and three barrows). Pigs fed diets with 3000 ppm ZnO had a higher BW during the overall period and ADG during d 8-21, d 22-42, and overall period than pigs receiving 300 ppm ZnO diets (p < 0.05), as well as a G: F which tended to increase on d 8-21 and overall period (p < 0.1) and decreased tendency on faecal gas emission of methyl mercaptans and acetic acid concentration (p < 0.1). Dietary probiotics complex supplementation had decreased the E. coli count (p < 0.05) and tended to increase the Lactobacillus count (p < 0.1). Dietary probiotics complex supplementation and different level of ZnO supplementation had no significant effect on the nutrition digestibility and faecal score (p > 0.05). In conclusion, probiotic supplementation reduced the fecal E. coli counts and tended to improve Lactobacillus counts. There were no interactive effects between ZnO and probiotic complex supplementation on all the measured parameters.

Probiotic mixture (Bacillus subtilis and Bacillus licheniformis) a potential in-feed additive to improve broiler production efficiency, nutrient digestibility, caecal microflora, meat quality and to diminish hazardous odour emission.

This research aimed to assess the impact of probiotic supplementation in the broiler diet on growth performance, nutrient utilization, noxious gas emissions, excreta micromiota and meat quality. One thousand six hundred and twenty male Ross 380 broilers (one-day-old, body weight, 42 ± 0.5 g and 5-week trial) were arbitrarily chosen and assigned to three nutritive treatments (basal diet and basal diet included with 0.1%, and 0.2% probiotic mixture [Bacillus subtilis 7.0 × 107 cfu/g, Bacillus licheniformis 4.1 × 107 cfu/g]) with 30 duplicates (18 birds each). Probiotic inclusion linearly increased (p < 0.05) broiler body weight gain (BWG) during Phases 1, 2 and the overall period and decreased (p < 0.05) feed conversion ratio (FCR) linearly on Phase 2 and the overall period. However, feed intake (FI) and mortality rate remained unaffected (p > 0.05). Though nutrient digestibility of nitrogen (N) tendency to increase (p < 0.05), dry matter (DM) and energy (E) did not influence (p > 0.05). Inclusion of a probiotic supplement linearly increased (p < 0.05) Lactobacillus and reduced Salmonella (p < 0.05) counts in broilers. Moreover, broilers fed a diet supplement with probiotic addition linearly decreased (p < 0.05) NH3 , H2 S, C2 O and acetic acid emissions. The graded level of probiotic addition linearly reduced (p < 0.05) cooking loss and the tendency to decrease (p < 0.05) weight of bursa of Fabricius, but had no effect (p > 0.05) on other meat quality measures. These findings indicated that increasing the level of probiotics in feed could improve growth efficiency, nutrient absorption, microbial index, meat quality and reduce gas emissions in broilers.

Effect of DHA supplementation on broilers' growth performance, meat quality and blood profile.

Docosahexaenoic acid (DHA) is vital in the meat composition of broilers which is helpful to maintain a healthy diet in humans. The purpose of this study is to observe the effect of DHA from fish oil on growth performance, organ weight, and meat quality along with the blood profile of broilers. A total of 1600, 1-day old, Ross 308 broilers with an average body weight of 43.15 ± 1.35 g were used in 5 weeks trail. Birds were randomly allotted into one of four dietary treatment groups named: control (CON), basal diet; treatment (TRT)1, CON + 0.05% DHA; TRT2, CON + 0.1% DHA; TRT3, CON + 0.15% DHA. Each treatment group consisted of 20 replications with 20 broilers per pen. The experiment was divided into three phases. Phase 1 (Day 1-7), Phase 2 (Day 7-21), and overall phase (Day 21-35). During Phase 1 and 2, no significant changes were observed on body weight gain (BWG) as the DHA levels increased in the diet. However, during Phase 3, broilers fed diet supplemented with DHA at increasing levels linearly increased (p < 0.05) BWG and feed intake (FI). Also, water holding capacity was reduced and drip loss percentage on Day 7 was increased linearly (p < 0.05) with the increase in DHA supplementation. At the end of trial, pH value of the meat was increased linearly (p < 0.05) with DHA levels. Moreover, blood cholesterol level, high-density lipoprotein, low-density lipoprotein was significantly and linearly (p < 0.05) decreased in DHA supplemented groups. Broiler-fed diet with DHA supplement decreased the red blood cell linearly (p < 0.05) in blood. The results of the study indicate that DHA supplementation increased BWG with FI. Moreover, it has reduced blood lipid constituents with some adverse effect on meat quality.

Effect of purified fish oil supplementation on growth performance, meat quality and blood profile in broilers.

The present research experimented with the view of observing the effect of supplementing purified fish oil (FO) on growth performance, organ weight, meat quality, along with blood profile of broilers. The experiment was conducted upon 1600 male Ross 308, each aged 1 day, broiler strains with an average body weight of 43.15 ± 1.35 g were randomly allotted into four dietary treatment groups where CON, basal diet; TRT1, CON + 0.05% FO; TRT2, CON + 0.10% FO; and TRT3, CON + 0.15% FO respectively. Each treatment group consisted of 20 replication pens, with each pen containing 20 broiler chicks. The 4-week long experiment was divided into two phases. The first phase (Days 7-21), the second phase (Days 22-35) and the overall (Days 7-35). On Day 21, the body weight of broilers in TRT2 and TRT3 was higher than the control. Increasing doses of purified FO supplementation showed a linear (p < 0.05) increase in body weight on Day 21. At the end of Phase 1, FO supplementation improved the body weight gain (BWG) of TRT2 and TRT3 group birds compared to CON. Increasing doses of FO supplementation increased the BWG linearly in all phases. Increasing doses of FO increased chicks' breast muscle weight with the highest value in the TRT3 group. Moreover, liver weight was linearly decreased in FO supplementation groups compared to the CON group. Abdominal fat was linearly decreased in FO supplemented groups. FO supplementation caused the lowest cholesterol and triglyceride in TRT3 than the CON group with a linear reduction. Low density lipoprotein (LDL) cholesterol was reduced by FO supplemental groups. In addition, FO supplementation increased the red blood cell (RBC) in the TRT3 group compared to the CON group. In conclusion, refined FO increased BWG, breast muscle weight and RBC and reduced cholesterol, triglycerides and LDL in broilers' blood.

Influence of Achyranthes japonica extracts supplementation in the diets of finishing pigs.

BACKGROUND: Herbs and their extracts have been used for a long time in animal industries as alternatives to an antibiotic. OBJECTIVES: This study was evaluated the effects of dietary supplementation of Achyranthes japonica extracts (AJE) on the performance and production parameters in finishing pigs. METHODS: Totally, 100 pigs with an average body weight of 50.33 ± 4.61 kg were used as a 10-week feeding trial. Pigs (five replicates, three barrows and two gilts per pen) were allotted randomly to four treatments as the addition of 0%, 0.05%, 0.10% and 0.20% of AJE in basal diets. RESULTS: A linear increase (p < .05) in average daily gain was observed during week 5, week 10 and overall period, and a linear decrease (p < .05) was observed in the feed conversion ratio during week 5. A linear increase (p < .05) in dry matter, protein digestibility and faecal ammonia emission on week 5 and week 10 and a linear increase (p < .05) in serum total protein concentration on week 10 of pigs fed diets supplemented with graded levels of AJE was observed. Faecal lactic acid bacteria counts showed a linear increase (p < .05) on week 5 with the increasing levels of AJE. CONCLUSIONS: In conclusion, there existed improvements in growth performance, nutrients digestibility, serum total protein, faecal coliform bacteria and lactic acid bacteria counts and faecal ammonia emission in the finishing pigs fed with AJE-supplemented diet.